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B2787
Sigma
Biotinylated Molecular Weight Marker
mol wt 6,500-180,000 Da
Description
| Components | This product contains: • α2-macroglobulin (human plasma) • β-galactosidase (E. coli) • phosphorylase b (rabbit muscle) • catalase (bovine liver) • alcohol dehydrogenase (equine liver) • carbonic anhydrase (bovine erythrocyte) • trypsin inhibitor (soy bean) • lysozyme (chicken egg) • aprotinin (bovine lung) Each vial contains approximately 33% protein, 33% NaCl and 33% sucrose. |
| Application | For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins.1 Visualization is accomplished using streptavidin-peroxidase (Cat. No. S5512) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli2 or the Weber and Osborn3, SDS-PAGE procedures. |
| Other Notes | Contains nine biotinylated proteins (approx 0.1 mg total). |
| Reconstitution | Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C. |
Properties
| form | lyophilized powder |
| usage | vial sufficient for 200 applications (on 10 cm × 10 cm mini gels) |
| mol wt | mol wt 6,500-180,000 Da |
| packaging | vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose) |
| storage temp. | −20°C |
Safety
| Hazard Codes | B |
| WGK Germany | 2 |
References
| Cited Reference | 1. Della-Penna, D., Christoffersen, R., Bennett, A.,, Biotinylated proteins as molecular weight standards on western blots. Anal. Biochem. 152, 329-332, (1986) Abstract |
| 2. Laemmli, U.K., Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680-685, (1970) Abstract | |
| 3. Weber, K. and Osborn, M., The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J. Biol. Chem. 244, 4406-4412, (1969) Abstract |
